Journal: Cells

Article Title: Regulatory Effects of Senescent Mesenchymal Stem Cells: Endotheliocyte Reaction

doi: 10.3390/cells13161345

Figure Lengend Snippet: Evaluation of senescence-associated secretory phenotype (SASP) in conditioned medium (CM) of “young” (MmC−) and senescent (MmC+) MSCs: ( a ) representative image of the dot blot (Human XL Cytokine Antibody Array Kit) and protein production. Densitometric analysis of certain analytes in antibody arrays was quantified using ImageLab software 5.0 (Bio-Rad). ( b ) Multiplexed fluorescent bead-based immunoassay detection of cytokines in CM using Human Cytokine/Chemokine (48-plex) panel. Data are shown as mean ± SD; n ≥ 4, * p < 0.05. MmC—mitomycin C, MSCs—mesenchymal stem cells.

Article Snippet: Forty-eight cytokines were measured using multiplexed fluorescent bead-based immunoassay detection (MILLIPLEX ® MAP system, Merck Millipore, Darmstadt, Germany) according to the manufacturer’s instructions with a Human Cytokine/Chemokine/Growth Factor Panel A—Immunology Multiplex Assay.

Techniques: Dot Blot, Ab Array, Software, Bead-based Assay

Journal: Cells

Article Title: Regulatory Effects of Senescent Mesenchymal Stem Cells: Endotheliocyte Reaction

doi: 10.3390/cells13161345

Figure Lengend Snippet: Cytokine concentration in conditioned medium of ECs and MSCs in monoculture, ECs cocultured with MSCs and ECs under conditioned medium from MSCs, evaluated using multiplexed fluorescent bead-based immunoassay. Data are shown as mean ± SD; n ≥ 4, * p < 0.05. MmC—mitomycin C, MSCs—mesenchymal stem cells, ECs—endothelial cells, EC control—monoculture of ECs (untreated), MSC control—monoculture of MSCs (MmC− and MmC+), ECs + MSCs—coculture, ECs + CM of MSCs—CM-treated ECs.

Article Snippet: Forty-eight cytokines were measured using multiplexed fluorescent bead-based immunoassay detection (MILLIPLEX ® MAP system, Merck Millipore, Darmstadt, Germany) according to the manufacturer’s instructions with a Human Cytokine/Chemokine/Growth Factor Panel A—Immunology Multiplex Assay.

Techniques: Concentration Assay, Bead-based Assay, Control

Journal: Ontario Health Technology Assessment Series

Article Title: Carrier Screening Programs for Cystic Fibrosis, Fragile X Syndrome, Hemoglobinopathies and Thalassemia, and Spinal Muscular Atrophy: A Health Technology Assessment

doi:

Figure Lengend Snippet: Characteristics of Included Studies

Article Snippet: Peyser et al, 2019 51 , United States , Noncomparative, retrospective , June 2013 to July 2015 (2 y) , ECS (CF, FXS, HbP, SMA) , PC , Individuals or couples seen at fertility centre during initial visit , 4,232 (1,206 couples) , Sequential and concurrent , NGS-based Counsyl ECS (400 variants of 102 genes).

Techniques: Sequencing, Variant Assay, Electrophoresis, Multiplex Assay, Next-Generation Sequencing, Microarray, Dot Blot, Hybridization, DNA Sequencing, Amplification, Long Range PCR, Marker, Northern Blot, Chromatography, Biomarker Discovery

Journal: Scientific Reports

Article Title: A method for reproducible measurements of serum BDNF: comparison of the performance of six commercial assays

doi: 10.1038/srep17989

Figure Lengend Snippet: BDNF ELISA Kits.

Article Snippet: Serum levels of total BDNF were measured by using six different ELISA kits, as listed in : human BDNF ELISA Kit (Cat #: SK00752-01, Aviscera-Bioscience, Santa Clara, CA, USA), BDNF Rapid TM ELISA Kit: Human, Mouse, Rat (2 Plates; Cat #: BEK-2211-2P, Biosensis Pty Ltd., SA, Australia), ChemKine TM BDNF Sandwich ELISA (Cat #: CYT306) and Milliplex ® Map Human Pituitary Magnetic Bead Panel 2 - Endocrine Multiplex Assay, based on Luminex ® /xMAP ® technology (Cat #: HPTP2MAG-66K, both from EMD Millipore Corporation, Billerica, MA, USA), BDNF Emax ® Immuno-Assay System (Cat #: G7610, Promega Corporation, Madison, WI, USA), Quantikine ® human BDNF Immunoassay (Cat #: DBD00, R&D Systems, Inc., Minneapolis, MN, USA).

Techniques: Enzyme-linked Immunosorbent Assay, Immuno Assay, Sandwich ELISA, Luminex, Recombinant, Magnetic Beads

Journal: Scientific Reports

Article Title: A method for reproducible measurements of serum BDNF: comparison of the performance of six commercial assays

doi: 10.1038/srep17989

Figure Lengend Snippet: Box plot of serum BDNF concentrations (ng/ml) from healthy volunteers (n = 38–40, see ) represented as mean of two independent measures. The upper line of the box marks the 75th percentile, the middle line is the median value and the lower line specifies the 25th percentile. Whiskers above and below the box indicate the 90th and 10th percentiles, respectively. Dots indicate the outlier values within each group.

Article Snippet: Serum levels of total BDNF were measured by using six different ELISA kits, as listed in : human BDNF ELISA Kit (Cat #: SK00752-01, Aviscera-Bioscience, Santa Clara, CA, USA), BDNF Rapid TM ELISA Kit: Human, Mouse, Rat (2 Plates; Cat #: BEK-2211-2P, Biosensis Pty Ltd., SA, Australia), ChemKine TM BDNF Sandwich ELISA (Cat #: CYT306) and Milliplex ® Map Human Pituitary Magnetic Bead Panel 2 - Endocrine Multiplex Assay, based on Luminex ® /xMAP ® technology (Cat #: HPTP2MAG-66K, both from EMD Millipore Corporation, Billerica, MA, USA), BDNF Emax ® Immuno-Assay System (Cat #: G7610, Promega Corporation, Madison, WI, USA), Quantikine ® human BDNF Immunoassay (Cat #: DBD00, R&D Systems, Inc., Minneapolis, MN, USA).

Techniques:

Journal: Scientific Reports

Article Title: A method for reproducible measurements of serum BDNF: comparison of the performance of six commercial assays

doi: 10.1038/srep17989

Figure Lengend Snippet: BDNF ELISA kits performance.

Article Snippet: Serum levels of total BDNF were measured by using six different ELISA kits, as listed in : human BDNF ELISA Kit (Cat #: SK00752-01, Aviscera-Bioscience, Santa Clara, CA, USA), BDNF Rapid TM ELISA Kit: Human, Mouse, Rat (2 Plates; Cat #: BEK-2211-2P, Biosensis Pty Ltd., SA, Australia), ChemKine TM BDNF Sandwich ELISA (Cat #: CYT306) and Milliplex ® Map Human Pituitary Magnetic Bead Panel 2 - Endocrine Multiplex Assay, based on Luminex ® /xMAP ® technology (Cat #: HPTP2MAG-66K, both from EMD Millipore Corporation, Billerica, MA, USA), BDNF Emax ® Immuno-Assay System (Cat #: G7610, Promega Corporation, Madison, WI, USA), Quantikine ® human BDNF Immunoassay (Cat #: DBD00, R&D Systems, Inc., Minneapolis, MN, USA).

Techniques: Enzyme-linked Immunosorbent Assay, Intra Assay, Inter Assay

Journal: Scientific Reports

Article Title: A method for reproducible measurements of serum BDNF: comparison of the performance of six commercial assays

doi: 10.1038/srep17989

Figure Lengend Snippet: Scatter plot showing the BDNF values distribution measured by the same operator on two different days using two plates of the same lot for each brand (Day 1 & Day 2). Each dot represents a BDNF value from one subject and the dashed lines link together two assessments of the same subject. The reproducibility was checked performing one-way ANOVA for repeated measures and the P values are specified.

Article Snippet: Serum levels of total BDNF were measured by using six different ELISA kits, as listed in : human BDNF ELISA Kit (Cat #: SK00752-01, Aviscera-Bioscience, Santa Clara, CA, USA), BDNF Rapid TM ELISA Kit: Human, Mouse, Rat (2 Plates; Cat #: BEK-2211-2P, Biosensis Pty Ltd., SA, Australia), ChemKine TM BDNF Sandwich ELISA (Cat #: CYT306) and Milliplex ® Map Human Pituitary Magnetic Bead Panel 2 - Endocrine Multiplex Assay, based on Luminex ® /xMAP ® technology (Cat #: HPTP2MAG-66K, both from EMD Millipore Corporation, Billerica, MA, USA), BDNF Emax ® Immuno-Assay System (Cat #: G7610, Promega Corporation, Madison, WI, USA), Quantikine ® human BDNF Immunoassay (Cat #: DBD00, R&D Systems, Inc., Minneapolis, MN, USA).

Techniques:

Journal: Scientific Reports

Article Title: A method for reproducible measurements of serum BDNF: comparison of the performance of six commercial assays

doi: 10.1038/srep17989

Figure Lengend Snippet: Scatter plots show the BDNF values distribution measured by the same operator on three different days for each brand. Distributions for Day 1 and Day 2 are the same as , here reported for comparison; distributions for Day 3 were obtained 1 year after Day 2, using a plate of a different lot (serum samples were stored at −80 °C). The reproducibility was checked performing one-way ANOVA for repeated measures and post-hoc Bonferroni correction applied when a significant comparison was found. P values and cumulative CVs expressed in percentage are given.

Article Snippet: Serum levels of total BDNF were measured by using six different ELISA kits, as listed in : human BDNF ELISA Kit (Cat #: SK00752-01, Aviscera-Bioscience, Santa Clara, CA, USA), BDNF Rapid TM ELISA Kit: Human, Mouse, Rat (2 Plates; Cat #: BEK-2211-2P, Biosensis Pty Ltd., SA, Australia), ChemKine TM BDNF Sandwich ELISA (Cat #: CYT306) and Milliplex ® Map Human Pituitary Magnetic Bead Panel 2 - Endocrine Multiplex Assay, based on Luminex ® /xMAP ® technology (Cat #: HPTP2MAG-66K, both from EMD Millipore Corporation, Billerica, MA, USA), BDNF Emax ® Immuno-Assay System (Cat #: G7610, Promega Corporation, Madison, WI, USA), Quantikine ® human BDNF Immunoassay (Cat #: DBD00, R&D Systems, Inc., Minneapolis, MN, USA).

Techniques: Comparison

Journal: Scientific Reports

Article Title: A method for reproducible measurements of serum BDNF: comparison of the performance of six commercial assays

doi: 10.1038/srep17989

Figure Lengend Snippet: ( A ) The antibodies from each ELISA kit were tested for specificity against pro-BDNF or mature BDNF. The BDNF standards blotted were commercial pro-BDNF (Alomone; 10 pg/lane), mature BDNF (1 and 2 from Alomone and Sigma, respectively; both 1000 pg/lane) and the standard BDNF protein included in each kit (Aviscera-Bioscience and Biosensis: 10 pg/lane; Millipore-ChemiKine TM , Millipore-Milliplex ® - and R&D System-Quantikine ® : 100 pg/lane; Promega-Emax ® : 1000 pg/lane). BSA (1000 pg/lane) was used as a negative control. The mouse monoclonal anti-BDNF antibody, (1:1000; Sigma) was tested as a control. ( B ) Central region of the same blot shown in A, from an overexposed film to better visualize the reactivity against pro-BDNF. ( C ) Reactivity of antibodies from Biosensis, Promega-Emax ® pAb and Sigma on a dot blot in which the same quantity of pro-BNDF and mature BDNF were spotted (100 pg each). Each antibody from the ELISA kits was used at the dilution suggested by the manufacturer’s instructions. mAb: Promega-Emax ® monoclonal capture antibody for plate coating. pAb: Promega-Emax ® polyclonal detection antibody.

Article Snippet: Serum levels of total BDNF were measured by using six different ELISA kits, as listed in : human BDNF ELISA Kit (Cat #: SK00752-01, Aviscera-Bioscience, Santa Clara, CA, USA), BDNF Rapid TM ELISA Kit: Human, Mouse, Rat (2 Plates; Cat #: BEK-2211-2P, Biosensis Pty Ltd., SA, Australia), ChemKine TM BDNF Sandwich ELISA (Cat #: CYT306) and Milliplex ® Map Human Pituitary Magnetic Bead Panel 2 - Endocrine Multiplex Assay, based on Luminex ® /xMAP ® technology (Cat #: HPTP2MAG-66K, both from EMD Millipore Corporation, Billerica, MA, USA), BDNF Emax ® Immuno-Assay System (Cat #: G7610, Promega Corporation, Madison, WI, USA), Quantikine ® human BDNF Immunoassay (Cat #: DBD00, R&D Systems, Inc., Minneapolis, MN, USA).

Techniques: Enzyme-linked Immunosorbent Assay, Negative Control, Control, Dot Blot